96 well plates (Revvity)

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Revvity 96 well plates
96 Well Plates, supplied by Revvity, used in various techniques. Bioz Stars score: 91/100, based on 27 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cellcarrier+ultra+microplates/CellCarrier-96+Ultra+Microplates%2C+Collagen-coated%2C+black%2C+96-well+with+lid%2C+case+of+40/pmc10133763__mmc5-247-31-33
Average 91 stars, based on 27 article reviews

96 well plates - by Bioz Stars, 2026-10

91/100 stars

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Electron Microscopy:

Article Title: FALCON systematically interrogates free fatty acid biology and identifies a novel mediator of lipotoxicity
Article Snippet: The primary antibodies used for staining were CMIP: (12851-1-AP, Proteintech) and PI3K p85L (#4292, Cell Signaling Technology). .. MIN6 cells grown on 384-well CellCarrier Ultra microplates (#6057308, PerkinElmer) were fixed for 10 min in PBS containing 4% PFA (Electron Microscopy Sciences), permeabilized for 15 min in 0.5% Triton X-100 (Sigma-Aldrich), blocked for 1 h in blocking reagent (100 mM Tris HCL pH 8; 150 mM NaCL; 5 g/L Blocking Reagent (#11096176001, Roche)) and treated for 1.5 h with primary antibody diluted in blocking reagent (NF-κB p65/RELA, Rabbit monoclonal antibody, 1:200, #8242, Cell Signaling Technology). .. Cells were washed three times in PBS and incubated for 0.5 h with fluorescent-labeled secondary antibody in blocking solution (1:500, Alexa Fluor 568 Goat anti-Rabbit IgG, (#A11036, Thermo Fisher Scientific)).

Article Title: FALCON systematically interrogates free fatty acid biology and identifies a novel mediator of lipotoxicity
Article Snippet: The primary antibodies used for staining were CMIP: (12851–1-AP, Proteintech) and PI3K p85ɑ: (#4292, Cell Signaling Technology). .. MIN6 cells grown on 384-well CellCarrier Ultra microplates (#6057308, PerkinElmer) were fixed for 10 min in PBS containing 4% PFA (Electron Microscopy Sciences), permeabilized for 15 min in 0.5% Triton X-100 (Sigma-Aldrich), blocked for 1 h in blocking reagent (100 mM Tris HCL pH 8; 150 mM NaCL; 5 g/L Blocking Reagent (#11096176001, Roche)) and treated for 1.5 h with primary antibody diluted in blocking reagent (NF-κB p65/RELA, Rabbit monoclonal antibody, 1:200, #8242, Cell Signaling Technology). .. Cells were washed three times in PBS and incubated for 0.5 h with fluorescent-labeled secondary antibody in blocking solution (1:500, Alexa Fluor 568 Goat anti-Rabbit IgG, (#A11036, Thermo Fisher Scientific)).

Blocking Assay:

Article Title: FALCON systematically interrogates free fatty acid biology and identifies a novel mediator of lipotoxicity
Article Snippet: The primary antibodies used for staining were CMIP: (12851–1-AP, Proteintech) and PI3K p85ɑ: (#4292, Cell Signaling Technology). .. MIN6 cells grown on 384-well CellCarrier Ultra microplates (#6057308, PerkinElmer) were fixed for 10 min in PBS containing 4% PFA (Electron Microscopy Sciences), permeabilized for 15 min in 0.5% Triton X-100 (Sigma-Aldrich), blocked for 1 h in blocking reagent (100 mM Tris HCL pH 8; 150 mM NaCL; 5 g/L Blocking Reagent (#11096176001, Roche)) and treated for 1.5 h with primary antibody diluted in blocking reagent (NF-κB p65/RELA, Rabbit monoclonal antibody, 1:200, #8242, Cell Signaling Technology). .. Cells were washed three times in PBS and incubated for 0.5 h with fluorescent-labeled secondary antibody in blocking solution (1:500, Alexa Fluor 568 Goat anti-Rabbit IgG, (#A11036, Thermo Fisher Scientific)).

Transfection:

Article Title: Hsc70-4 mediates internalization of environmental dsRNA at the surface of Drosophila S2 cells
Article Snippet: .. Cells were seeded on black CellCarrier Ultra microplates (6055302, PerkinElmer) and transfected with 10 ng/well of dsRNA targeting the candidates using the Effectene Transfection reagent (301427, QIAGEN). dsCtl and Dynasore (D7693, Sigma-Aldrich) wells were transfected with dsCtl (dsFLuc). ..

Immunofluorescence:

Article Title: A seeding-based neuronal model of tau aggregation for use in drug discovery
Article Snippet: .. For use in immunofluorescence (IF), cells were plated in tissue culture treated black 96-well plates with optically clear bottom (CellCarrier Ultra microplates, PerkinElmer). ..

IF-cells:

Incubation:

Article Title: Emodin, an Emerging Mycotoxin, Induces Endoplasmic Reticulum Stress-Related Hepatotoxicity through IRE1α–XBP1 Axis in HepG2 Cells
Article Snippet: The IC 50 value was calculated using GraphPad Prism software (version 8.0.2; San Diego, CA, USA). .. HepG2 cells were seeded in collagen-coated CellCarrier Ultra microplates (PerkinElmer, Waltham, MA, USA) at a density of 2.0 × 10 4 cells/well, and incubated for 24 h. Subsequently, cells were treated with 25, 50, 100, and 200 μM of emodin for 24 h. Emodin was dissolved in DMSO, and the final concentration of DMSO in the medium was maintained at 0.5% ( v / v ). .. PBS was used to rinse the cells, and 4% paraformaldehyde (FUJIFILM Wako Pure Chemical Corporation, Osaka, Japan) was used for 20 min to fix the cells.

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96 well plates (Revvity)

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