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96 well plates  (Revvity)


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    Revvity 96 well plates
    96 Well Plates, supplied by Revvity, used in various techniques. Bioz Stars score: 91/100, based on 27 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cellcarrier+ultra+microplates/CellCarrier-96+Ultra+Microplates%2C+Collagen-coated%2C+black%2C+96-well+with+lid%2C+case+of+40/pmc10133763__mmc5-247-31-33
    Average 91 stars, based on 27 article reviews
    96 well plates - by Bioz Stars, 2026-10
    91/100 stars

    Images

    Related Articles

    Electron Microscopy:

    Article Title: FALCON systematically interrogates free fatty acid biology and identifies a novel mediator of lipotoxicity
    Article Snippet: The primary antibodies used for staining were CMIP: (12851-1-AP, Proteintech) and PI3K p85L (#4292, Cell Signaling Technology). .. MIN6 cells grown on 384-well CellCarrier Ultra microplates (#6057308, PerkinElmer) were fixed for 10 min in PBS containing 4% PFA (Electron Microscopy Sciences), permeabilized for 15 min in 0.5% Triton X-100 (Sigma-Aldrich), blocked for 1 h in blocking reagent (100 mM Tris HCL pH 8; 150 mM NaCL; 5 g/L Blocking Reagent (#11096176001, Roche)) and treated for 1.5 h with primary antibody diluted in blocking reagent (NF-κB p65/RELA, Rabbit monoclonal antibody, 1:200, #8242, Cell Signaling Technology). .. Cells were washed three times in PBS and incubated for 0.5 h with fluorescent-labeled secondary antibody in blocking solution (1:500, Alexa Fluor 568 Goat anti-Rabbit IgG, (#A11036, Thermo Fisher Scientific)).

    Article Title: FALCON systematically interrogates free fatty acid biology and identifies a novel mediator of lipotoxicity
    Article Snippet: The primary antibodies used for staining were CMIP: (12851–1-AP, Proteintech) and PI3K p85ɑ: (#4292, Cell Signaling Technology). .. MIN6 cells grown on 384-well CellCarrier Ultra microplates (#6057308, PerkinElmer) were fixed for 10 min in PBS containing 4% PFA (Electron Microscopy Sciences), permeabilized for 15 min in 0.5% Triton X-100 (Sigma-Aldrich), blocked for 1 h in blocking reagent (100 mM Tris HCL pH 8; 150 mM NaCL; 5 g/L Blocking Reagent (#11096176001, Roche)) and treated for 1.5 h with primary antibody diluted in blocking reagent (NF-κB p65/RELA, Rabbit monoclonal antibody, 1:200, #8242, Cell Signaling Technology). .. Cells were washed three times in PBS and incubated for 0.5 h with fluorescent-labeled secondary antibody in blocking solution (1:500, Alexa Fluor 568 Goat anti-Rabbit IgG, (#A11036, Thermo Fisher Scientific)).

    Blocking Assay:

    Article Title: FALCON systematically interrogates free fatty acid biology and identifies a novel mediator of lipotoxicity
    Article Snippet: The primary antibodies used for staining were CMIP: (12851-1-AP, Proteintech) and PI3K p85L (#4292, Cell Signaling Technology). .. MIN6 cells grown on 384-well CellCarrier Ultra microplates (#6057308, PerkinElmer) were fixed for 10 min in PBS containing 4% PFA (Electron Microscopy Sciences), permeabilized for 15 min in 0.5% Triton X-100 (Sigma-Aldrich), blocked for 1 h in blocking reagent (100 mM Tris HCL pH 8; 150 mM NaCL; 5 g/L Blocking Reagent (#11096176001, Roche)) and treated for 1.5 h with primary antibody diluted in blocking reagent (NF-κB p65/RELA, Rabbit monoclonal antibody, 1:200, #8242, Cell Signaling Technology). .. Cells were washed three times in PBS and incubated for 0.5 h with fluorescent-labeled secondary antibody in blocking solution (1:500, Alexa Fluor 568 Goat anti-Rabbit IgG, (#A11036, Thermo Fisher Scientific)).

    Article Title: FALCON systematically interrogates free fatty acid biology and identifies a novel mediator of lipotoxicity
    Article Snippet: The primary antibodies used for staining were CMIP: (12851–1-AP, Proteintech) and PI3K p85ɑ: (#4292, Cell Signaling Technology). .. MIN6 cells grown on 384-well CellCarrier Ultra microplates (#6057308, PerkinElmer) were fixed for 10 min in PBS containing 4% PFA (Electron Microscopy Sciences), permeabilized for 15 min in 0.5% Triton X-100 (Sigma-Aldrich), blocked for 1 h in blocking reagent (100 mM Tris HCL pH 8; 150 mM NaCL; 5 g/L Blocking Reagent (#11096176001, Roche)) and treated for 1.5 h with primary antibody diluted in blocking reagent (NF-κB p65/RELA, Rabbit monoclonal antibody, 1:200, #8242, Cell Signaling Technology). .. Cells were washed three times in PBS and incubated for 0.5 h with fluorescent-labeled secondary antibody in blocking solution (1:500, Alexa Fluor 568 Goat anti-Rabbit IgG, (#A11036, Thermo Fisher Scientific)).

    Transfection:

    Article Title: Hsc70-4 mediates internalization of environmental dsRNA at the surface of Drosophila S2 cells
    Article Snippet: .. Cells were seeded on black CellCarrier Ultra microplates (6055302, PerkinElmer) and transfected with 10 ng/well of dsRNA targeting the candidates using the Effectene Transfection reagent (301427, QIAGEN). dsCtl and Dynasore (D7693, Sigma-Aldrich) wells were transfected with dsCtl (dsFLuc). ..

    Immunofluorescence:

    Article Title: A seeding-based neuronal model of tau aggregation for use in drug discovery
    Article Snippet: .. For use in immunofluorescence (IF), cells were plated in tissue culture treated black 96-well plates with optically clear bottom (CellCarrier Ultra microplates, PerkinElmer). ..

    IF-cells:

    Article Title: A seeding-based neuronal model of tau aggregation for use in drug discovery
    Article Snippet: .. For use in immunofluorescence (IF), cells were plated in tissue culture treated black 96-well plates with optically clear bottom (CellCarrier Ultra microplates, PerkinElmer). ..

    Incubation:

    Article Title: Emodin, an Emerging Mycotoxin, Induces Endoplasmic Reticulum Stress-Related Hepatotoxicity through IRE1α–XBP1 Axis in HepG2 Cells
    Article Snippet: The IC 50 value was calculated using GraphPad Prism software (version 8.0.2; San Diego, CA, USA). .. HepG2 cells were seeded in collagen-coated CellCarrier Ultra microplates (PerkinElmer, Waltham, MA, USA) at a density of 2.0 × 10 4 cells/well, and incubated for 24 h. Subsequently, cells were treated with 25, 50, 100, and 200 μM of emodin for 24 h. Emodin was dissolved in DMSO, and the final concentration of DMSO in the medium was maintained at 0.5% ( v / v ). .. PBS was used to rinse the cells, and 4% paraformaldehyde (FUJIFILM Wako Pure Chemical Corporation, Osaka, Japan) was used for 20 min to fix the cells.

    Concentration Assay:

    Article Title: Emodin, an Emerging Mycotoxin, Induces Endoplasmic Reticulum Stress-Related Hepatotoxicity through IRE1α–XBP1 Axis in HepG2 Cells
    Article Snippet: The IC 50 value was calculated using GraphPad Prism software (version 8.0.2; San Diego, CA, USA). .. HepG2 cells were seeded in collagen-coated CellCarrier Ultra microplates (PerkinElmer, Waltham, MA, USA) at a density of 2.0 × 10 4 cells/well, and incubated for 24 h. Subsequently, cells were treated with 25, 50, 100, and 200 μM of emodin for 24 h. Emodin was dissolved in DMSO, and the final concentration of DMSO in the medium was maintained at 0.5% ( v / v ). .. PBS was used to rinse the cells, and 4% paraformaldehyde (FUJIFILM Wako Pure Chemical Corporation, Osaka, Japan) was used for 20 min to fix the cells.



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    Inhibition of ATM under autophagy-inducing conditions leads to cell death. (A) HeLa cells were seeded on <t>96-well</t> plates and treated with DMSO or 10 µM KU-55933 in 10% FBS-supplemented DMEM (control; normal medium), 1% FBS-supplemented DMEM or FBS-free DMEM. Images acquired by the IncuCyte instrument at the indicated times were analyzed using ZOOM 2016 software. Confluence was measured in triplicate wells. Values are means ± SEMs (Student’s t-test, *** p <0.001). (B) Protein extracts from HeLa cells treated as in (A) for 48 h were subjected to Western blotting using the indicated antibodies. Values are ratios of LC3-II, p62, or cleaved PARP to β-actin densitometry values (control values set to 1). Values are means ± SEMs (Student’s t-test, *** p <0.01). (C) HeLa cells were treated with DMSO or 10 µM KU-55933 in 10% FBS-supplemented DMEM (control) or 1% FBS-supplemented DMEM for 24 h. The cells were fixed and processed for immunofluorescence using an anti-LC3 antibody. LC3 puncta in the cytoplasm were analyzed using the Operetta CLS High Content Analysis System. (D) DNA fragmentation in cell lysates from HeLa cells treated as in (A) for 48 h was measured using the Cell Death Detection Kit (Roche). y-Axes, degree of DNA fragmentation. (E) HeLa cells were pretreated with 10 µM or 20 µM Q-VD-Oph for 1 h before treatment with DMSO in 10% FBS-supplemented DMEM (control) or 10 μM KU-55933 in 1% FBS-supplemented DMEM. After 48 h, cell viability was evaluated using the MTT assay. Data in (C), (D), and (E) are means ± SEMs of three independent experiments (Student’s t-test, *** p <0.001).
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    Image Search Results


    Inhibition of ATM under autophagy-inducing conditions leads to cell death. (A) HeLa cells were seeded on 96-well plates and treated with DMSO or 10 µM KU-55933 in 10% FBS-supplemented DMEM (control; normal medium), 1% FBS-supplemented DMEM or FBS-free DMEM. Images acquired by the IncuCyte instrument at the indicated times were analyzed using ZOOM 2016 software. Confluence was measured in triplicate wells. Values are means ± SEMs (Student’s t-test, *** p <0.001). (B) Protein extracts from HeLa cells treated as in (A) for 48 h were subjected to Western blotting using the indicated antibodies. Values are ratios of LC3-II, p62, or cleaved PARP to β-actin densitometry values (control values set to 1). Values are means ± SEMs (Student’s t-test, *** p <0.01). (C) HeLa cells were treated with DMSO or 10 µM KU-55933 in 10% FBS-supplemented DMEM (control) or 1% FBS-supplemented DMEM for 24 h. The cells were fixed and processed for immunofluorescence using an anti-LC3 antibody. LC3 puncta in the cytoplasm were analyzed using the Operetta CLS High Content Analysis System. (D) DNA fragmentation in cell lysates from HeLa cells treated as in (A) for 48 h was measured using the Cell Death Detection Kit (Roche). y-Axes, degree of DNA fragmentation. (E) HeLa cells were pretreated with 10 µM or 20 µM Q-VD-Oph for 1 h before treatment with DMSO in 10% FBS-supplemented DMEM (control) or 10 μM KU-55933 in 1% FBS-supplemented DMEM. After 48 h, cell viability was evaluated using the MTT assay. Data in (C), (D), and (E) are means ± SEMs of three independent experiments (Student’s t-test, *** p <0.001).

    Journal: Biomolecules & Therapeutics

    Article Title: Ataxia-Telangiectasia Mutated Is Involved in Autolysosome Formation

    doi: 10.4062/biomolther.2023.003

    Figure Lengend Snippet: Inhibition of ATM under autophagy-inducing conditions leads to cell death. (A) HeLa cells were seeded on 96-well plates and treated with DMSO or 10 µM KU-55933 in 10% FBS-supplemented DMEM (control; normal medium), 1% FBS-supplemented DMEM or FBS-free DMEM. Images acquired by the IncuCyte instrument at the indicated times were analyzed using ZOOM 2016 software. Confluence was measured in triplicate wells. Values are means ± SEMs (Student’s t-test, *** p <0.001). (B) Protein extracts from HeLa cells treated as in (A) for 48 h were subjected to Western blotting using the indicated antibodies. Values are ratios of LC3-II, p62, or cleaved PARP to β-actin densitometry values (control values set to 1). Values are means ± SEMs (Student’s t-test, *** p <0.01). (C) HeLa cells were treated with DMSO or 10 µM KU-55933 in 10% FBS-supplemented DMEM (control) or 1% FBS-supplemented DMEM for 24 h. The cells were fixed and processed for immunofluorescence using an anti-LC3 antibody. LC3 puncta in the cytoplasm were analyzed using the Operetta CLS High Content Analysis System. (D) DNA fragmentation in cell lysates from HeLa cells treated as in (A) for 48 h was measured using the Cell Death Detection Kit (Roche). y-Axes, degree of DNA fragmentation. (E) HeLa cells were pretreated with 10 µM or 20 µM Q-VD-Oph for 1 h before treatment with DMSO in 10% FBS-supplemented DMEM (control) or 10 μM KU-55933 in 1% FBS-supplemented DMEM. After 48 h, cell viability was evaluated using the MTT assay. Data in (C), (D), and (E) are means ± SEMs of three independent experiments (Student’s t-test, *** p <0.001).

    Article Snippet: Cells were seeded at 1×10 3 /well in a 96-well plate (Cell carrier-96-ultra, black wall and clear bottom, #6055302, PerkinElmer, Waltham, MA, USA) and subjected to the indicated conditions.

    Techniques: Inhibition, Software, Western Blot, Immunofluorescence, High Content Screening, MTT Assay

    Journal:

    Article Title:

    doi:

    Figure Lengend Snippet:

    Article Snippet: Cellcarrier ultra (PE) 384 imaging plates , PerkinElmer , CAT#6057302.

    Techniques: Recombinant, Membrane, Expressing, Plasmid Preparation, Clone Assay, Software, Cell Culture, Imaging, Microscopy, High Content Screening, Transferring